FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2717366
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGC14150.022.7555318
CCTTGCG9600.021.512579
TAGGTAG4800.018.2286435
GACGCGC3850.018.182338
CCCCTAG2701.2369128E-1018.151971
CTACCCC3750.017.7328264
CGCGGGA2651.886292E-917.1693256
TCCGCAC5000.016.80013764
CGTCCGG3551.8189894E-1216.76008432
AGGTAGC5250.016.666197
CCGCACC5400.016.20383565
GTCCTAC4000.015.7533171
CCGTCCG4000.015.74954931
CTAGGGT2452.4823748E-715.7138354
CCCTCGG3351.5825208E-1015.6711928
ACGCGCA4500.015.55596839
CCTTGCA11100.015.4519999
CTTAAGG6350.015.4329143
CTTCCTA11800.015.4269771
CGCGCAT5000.015.40040940
Produced by FastQC (version 0.11.5)