FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-C05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-C05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2045515
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTGAC1755.626885E-515.999147
TACACGG2854.953108E-915.9640545
TACACCG3302.1409505E-914.8476875
TCCTATA9550.014.660692
TCCGCAC3403.261448E-914.41134264
GTATTAG9550.013.9296981
CTATACG4051.8371793E-1013.8264174
TATTAGG8650.013.7581672
CTTACAC5100.013.7257593
CCGCACC3607.2886905E-913.61071165
TCCTTGC11600.013.5768578
GTAGGAC2607.89986E-613.4618013
TAGGTAG4951.8189894E-1213.433625
GGTAGTC5900.013.0501467
CGCACCT3507.391645E-812.99993666
ACTAGAC4651.200533E-1012.7959493
CTAATAC11000.012.7275223
GCGCACT3052.9291114E-612.62566811
TATACCG6100.012.6222725
TCCTACC7800.012.5649632
Produced by FastQC (version 0.11.5)