FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-B09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-B09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040824
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC8900.018.8756053
CTAGGAC2952.3646862E-1117.7958563
CCTAGCG2401.031367E-817.49925830
GTATTAG8950.017.2123011
TCCGCAC3950.015.94908164
CCGCACC4201.8189894E-1214.99973365
ACTATGC9100.014.9993658
TAGGTAG4600.014.4559115
CTAGACC5400.014.2586564
GGTAGTC5000.013.9994087
GTATAGA9900.013.7923631
GACTAGG3303.421701E-813.7872948
CGCACCT4607.2759576E-1213.69574466
TCGGGGG1550.00737906313.54781334
GTCGGGG1550.00737906313.54781333
CCTAATA10800.013.2867992
CAGCTCG1850.001548004813.2443049
TAGTCAG4506.91216E-1113.2232829
GTATTAT17250.013.1926971
TACATAG6650.013.1573385
Produced by FastQC (version 0.11.5)