FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP3-A02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP3-A02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291817
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC4000.019.2496464
CGCACCT4600.018.26052966
CCGCACC4450.018.0895565
CTAATAC11350.015.110513
TATACCG7300.014.8627375
GTATATG15450.014.7284991
CTAGGAC3504.9039954E-914.0003493
GTATTAG11350.013.8800271
TAGGTAG5150.013.5919795
TACACTG7400.013.2429955
ATCACAC8200.013.2314629
GTATATA23100.012.881921
ACTGCGC3558.899951E-812.8166628
AGTGTGC4406.87578E-1012.7270358
TCACACA6900.012.680922530
CTAGGGT3103.5235753E-612.4191234
TCTATAC9600.012.3961423
ACTATGC11450.012.2268468
TATACAG13750.012.2179545
TAATACT17800.012.19078354