FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP2-H05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP2-H05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260664
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT3803.6379788E-1215.65851566
CCTATAC7600.015.6567823
GCGCACT3101.5003025E-814.67962411
TAGACCG5100.014.4110585
GTATTAG10600.014.2011871
TCCGCAC4202.1827873E-1114.16691264
CCCTATA6200.014.1128372
ATGCGCG2001.7539944E-414.00086240
CTAATAC11300.013.627353
TAGACAG8250.013.5750925
CCGCACC4152.7102942E-1013.4945165
TATACAG15100.013.443055
CCTAGGC1600.00910390413.1240663
CTTAGAC5900.013.0499193
TAGGTAG5153.6379788E-1212.9119895
TCCTAGG2751.4085061E-512.7272132
GTATAAT18800.012.6623151
GTATATG15300.012.5844231
CCCTAGG1950.002301544912.5640432
TCCTATA9300.012.4192952
Produced by FastQC (version 0.11.5)