FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP2-G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP2-G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2001089
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC3700.017.97275464
CCGCACC3850.017.27251865
GTATTAG7500.015.8704391
GTATAGA9500.015.8458721
CGCACCT4151.8189894E-1215.18053966
TACACCG3251.724402E-915.0767395
CCCGTGC1400.00375551914.9998198
CTAGCAC5600.014.9998193
CGGGTCC2604.866306E-714.80751225
ACCCGGG1450.004740340614.48330711
GTATAAG8800.014.3215861
CCTAGCG2303.7333095E-513.69548630
TCTATAC9600.013.489423
CATATAC11750.013.4040933
GTGTAAG5750.013.3944891
CTAATAC8900.013.3706253
TACACAG6300.013.3331725
CAGTGCA5300.013.2080479
GCTAGCC2456.75539E-512.8569883
CGCGCAA2203.9301405E-412.72775410
Produced by FastQC (version 0.11.5)