FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP2-G02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP2-G02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2323505
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG5000.016.0989365
GTATTAG10250.014.6866981
GTATATA24650.014.4864781
CGGGGCA1750.001016520914.0035959
GTATAGA11050.013.940231
CGGGAGC4152.728484E-1013.49308418
GTCTATG6750.013.4849431
TAGGACA5800.013.2749844
GCTCGGT1900.001889741912.89804811
TATACGG7450.012.6837255
TATACCG7200.012.6380535
TACGCCG2508.166951E-512.5991675
CCCGTAC2852.0322828E-512.2809483
ATACGGT8850.012.2590776
GTCTAGG4902.8921932E-1012.1459751
TACTCCG3205.0267627E-612.0304555
ACCTAGG2050.003341756811.95428851
TAGACAG7650.011.8946395
CTAGTAC7950.011.8870313
TATACTG13850.011.87647155
Produced by FastQC (version 0.11.5)