FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP2-D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP2-D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779186
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGC800.00266872921.873576
CTGTGCG1602.6057738E-517.5022989
TATACCG4900.015.7132575
TATACTG12250.014.8561715
CCTATAC5050.014.5535033
ACGGTGT5350.014.3915828
GCGCACT1951.4134569E-414.36086111
GTATATA24250.014.2909381
TCCGCAC2951.3030512E-714.23715764
CCGCACC2951.3030512E-714.23715765
CCGCGCA1850.001547271913.2449829
GTATAGA9400.013.0339931
GTATTAG7650.012.81251
CTATACT8250.012.7264394
CATATAC12050.012.4888093
ATTATAC15250.012.3926323
CTAATAC8800.012.3287383
AGTCCGA2305.7105016E-412.17551110
TAGGAGT5501.2732926E-1112.0901174
CGCACCT3501.0398053E-612.00022766