FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP2-C12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP2-C12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022441
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG3150.026.66633254
CGCGGGA3550.023.66167456
GCTATAC8600.018.7203853
TCCTTGC13500.018.4065678
CGGGCCG4800.017.49978322
GTATCCT8850.017.4046551
GTCTCCT6650.017.371941
GCGCACC5150.016.98951535
ACGGGCC5550.016.3961921
CGTCCGG4700.016.3822332
CCGGAGC5550.015.76661340
AACGCGC5550.015.76556952
CCTTGCG10900.015.7350499
CTATACA12200.015.49109654
TCACGGG5950.015.2934219
CACGGGC5750.015.21720120
CTCCTAG3009.731593E-915.169741
CCCCTAT6100.014.9210551
CGCACCG5900.014.83130436
CTAATAC12850.014.7077443
Produced by FastQC (version 0.11.5)