FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP2-C10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP2-C10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2125230
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC9700.015.5148953
GTACTAG5100.015.102461
TACACCG4700.014.8930715
GTATAGA8700.014.8894171
CCTACAC4251.8189894E-1214.8229873
CGCACCT3604.656613E-1014.58348566
TACACAG7200.014.5827985
TCTATAC9150.014.53498653
TCCTAAG4051.8371793E-1013.826982
CTACACA6700.013.5815924
CCCGAGT1550.00737776513.5482161
ACTATGC8450.013.2539518
GGCGTCC2659.618892E-613.20737421
TATACAG12750.013.1759875
CTATACA9700.012.9892154
TAATACT15900.012.9869454
CTTACAC5400.012.9624893
AGCGCGT1900.001891814412.89608410
CCGCACC4355.7298166E-1012.87339565
GTATTAG8450.012.8439961
Produced by FastQC (version 0.11.5)