FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP2-B07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP2-B07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2212792
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG5000.016.0994435
CTTAGAC5400.015.555373
CTAATAC10700.015.3736483
TAGGACT3452.4556357E-1015.2168664
GTATTAG10000.015.0539021
TCTATAC9500.013.6314163
TACACCG4152.728484E-1013.4935095
TCCTAGG2901.6674239E-613.2760032
CATATAC12550.012.8285313
GTATAGG7650.012.8137791
ACTATGC9350.012.7268328
GTCTAAG6900.012.6844471
TATACAG13150.012.5090735
ACCGGGC2254.753296E-412.4440158
CCGCACC4802.055458E-1012.39596665
CGCACCT4255.5788405E-912.35307266
TATAGGA9650.012.3317372
GTCCTAA4852.4374458E-1012.2712221
TCCGCAC4601.3860699E-912.17404364
GTATATA21600.012.1559291
Produced by FastQC (version 0.11.5)