FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP2-B04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP2-B04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2533702
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG1356.0260136E-620.742089
ACGCGCA2104.274989E-818.33451739
GTATTAG11650.017.128611
GTATAGA11550.015.7613931
CGCGCAT2452.4795554E-715.71530140
TATACGG10500.014.9994895
GTCCTAC3403.250534E-914.4152561
ACTGCGT3951.2369128E-1014.1767328
CCGCACC4202.1827873E-1114.16702265
CGCGCAC1750.001018043614.00090310
CCTAGCG2759.1759284E-713.99952130
TCCGCAC4303.274181E-1113.83755664
GACCGCG1550.007379306513.5479257
GTCCTAA4455.820766E-1113.3740251
CTAATAC15450.013.3660313
CGTCGCG1850.001548365913.24409910
GTATTAT24650.013.0660451
CATATAC19400.012.9900173
CGCACCT4851.8189894E-1112.99001766
CCGCGCA1900.00189255812.8955699
Produced by FastQC (version 0.11.5)