FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP2-B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP2-B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1685268
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA8250.015.700071
GTATTAG6800.015.4442271
ACCGTGC2151.9772668E-514.65057858
TCGTGCG1951.4133051E-414.3609589
TAGACTG3901.549779E-913.4610015
TGCGTGG2354.562385E-513.40610910
GTATATA20000.013.3026281
CACGCAG3203.5129233E-713.12447716
ATCGTGC1600.00910076713.1244778
AGACCGC1950.002301731812.56360156
TATACAG11500.012.4777645
TAGACCG3651.2782584E-712.4652565
CAGGACT3701.5260957E-712.2968084
CCCGGCC2000.002783171612.24951218
GTATATG8900.012.193421
CTAGGAC2305.715602E-412.174153
GTGTAGG3751.8117316E-712.1357311
GTACTAA5206.002665E-1112.1177781
ACTGATC4409.335054E-911.9313428
GTAGACT4206.2700565E-811.6662034
Produced by FastQC (version 0.11.5)