FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641831
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGGCG1401.8644978E-417.5022019
CTACACG1653.3978955E-516.9692484
CGCGCAC1250.001761545516.80211310
GTGTAAG3601.8189894E-1216.528851
TATACGG5600.015.6245855
TAGGTAG3452.4556357E-1015.2169885
AGACCGC2352.7735368E-614.8932226
TACACTG5950.014.7054925
ACGGTGT5000.014.699618
ACTCCGC2403.4510522E-614.5829478
GTATTAG7000.014.500941
GACCGCG1708.1793783E-414.4113837
GTATATA16150.014.3043331
TCCTAAG3758.60382E-1014.0004812
CAGGACC2801.1234388E-613.7496354
CCTACAC2405.567704E-513.1246523
CTTACAC4304.765752E-1013.0229113
GTCTTAG3801.5521437E-812.8955731
TAGGACT2508.1611026E-512.5996664
TATACAG11400.012.5873855
Produced by FastQC (version 0.11.5)