FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-H08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-H08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9612961
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC15500.015.12915364
CCGCACC17050.014.36961765
GTATTAG42900.013.7093991
CTAATAC46150.013.5747723
TAGGTAG23200.013.1242865
TAGACAG30050.012.9277515
GTATATA94550.012.6258121
TATACAG57750.012.5447735
TATACTG56400.012.4106735
CGCACCT19200.012.39599866
CCTACAC16400.012.1641633
TAATACT80450.011.9633034
ACTATGC41250.011.7932998
TATACGG33250.011.4730625
GTATAGG33500.011.2861391
ACGGTGT27650.011.265218
GTATAGA43950.010.9922511
CGATCTA44150.010.9396170
CATATAC59450.010.8910833
ACCGTGC11250.010.8882968
Produced by FastQC (version 0.11.5)