FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-H03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-H03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCACCACAACCATCATCAAATAATTATTGATGAAATATGGGTTAATTA11.6129032258064515No Hit
CATATCACCTGCGTTTCCGTTAAACTATCGGTTGCGGCCATATCTACCAG11.6129032258064515No Hit
GTAATATATTTGATAAAAAATCACCAGGAGTCACACATGACTCCCGTATA11.6129032258064515No Hit
TTGATATATTGCCCCAAATATCAGGTAAAACAACAGTAAAATACCATAAA11.6129032258064515No Hit
CGAATAGATAGTCTTTTTCTATTTTCAGTAAATGTGTCATTGAAAAAAGT11.6129032258064515No Hit
CAGATGTGCAGATGTAACCAAAAACTTCACAGTTGTTTGCCATCTTAGTA11.6129032258064515No Hit
TTTATTATTCGAACTAAAATTGAATTGTAAAATCTTTAACGATCAGGCCA11.6129032258064515No Hit
ATACAAGAATATAAAAATAACGAACAACACAAACATCTATAAATCTATAG11.6129032258064515No Hit
GCCGTAGGAAGAGGGAAAAACGAACAGGGAAGAAGCAGGAAGAGCAGACA11.6129032258064515No Hit
ATACAAATACAGGTCGATAAATAACTACGGAGTGCTTGAGTAATAAAAGA11.6129032258064515No Hit
CATCATAGGTTACCTATCTCCGTTCTTCTAGAACCAAAAGAACAAAAATT11.6129032258064515No Hit
TGCCAGAATTCGTGAAAGCCCCGTTATTGATGTGATTAGCAAACTCGTCG11.6129032258064515No Hit
AAGTTGGTTTAAGGCGCAAGACTGTAATTTATCACTACGAAATCTTGAGA11.6129032258064515No Hit
ATGGATTTTTAGGTAAAAGAATTTGTCAAGAAGCAGTGACTTCGGGCTAC11.6129032258064515No Hit
TCACTGATATTCCTGTCGAGTACGGACCTGGGGGAGATATTGCCATTTCG11.6129032258064515No Hit
TCTCTTGTTGAATATTTACCAATTGGGAAAAAGAACTCGTATTTCATTCC11.6129032258064515No Hit
TTCTAAAATTATAGAATTCGCGTTAAAATGTCCGGGTTTCTCAGCACTTC11.6129032258064515No Hit
GCACAAATGAAGGCTGAGGGGACTAAACCAAATACAATCACTTTTAATCT11.6129032258064515No Hit
ATGTTTGGGGTTTCTGTTTAGTAAACCCTTTACAAAAGACCTCCCCTCTT11.6129032258064515No Hit
TCATTAACGAGGAAAAATGCCCTATTTTGTTTAGCAATTAATGAACCATC11.6129032258064515No Hit
AAGGAGAACAATAGCAAGAGGGCTTTTTAAATTTAAGACTTGTGCTCATC11.6129032258064515No Hit
ATATGATACAAGACACTTTTGAACTTTGTACGACGAATTTTGAGGTTCGC11.6129032258064515No Hit
GAATCCAGTGTGATATCAGCGGGAATACTATCACCCTGGTATAGCTCATA11.6129032258064515No Hit
GTGTTTCCAAATCAACGGTGAAAAAAATCTCGTATGAAGTCCAACACAGG11.6129032258064515No Hit
GATGAGAATGGATTGAACGAGATGAGAAATGAAGTGGAGGTAATGAAGAA11.6129032258064515No Hit
GAACAAACAGAATGGCAGGCTAATCCCTGCTTTATACGCTTTGATTGCCT11.6129032258064515No Hit
GTCCTGTTCCAAGGAACATAGACAAGGAACGGCCCCAAAGTTGCCCTCTC11.6129032258064515No Hit
TCTATGCCACGCTCACCAGTGATGATACTTGCGTCATCTTGATCGTCAAA11.6129032258064515No Hit
GCTTGGTTACGCGCCGTAGAACTTCATCTTAGGCATCAAGACCTATAATA11.6129032258064515No Hit
GTACTAGTCACATACCCGAGCTTCAAACAATTGACCAACGCCTATTTTAT11.6129032258064515No Hit
ATCAATATCGCTTGCTGTAAAACTAGAATTTACCAAACTTGAAGAGTTGT11.6129032258064515No Hit
TGATTGGACCCTGAGGTGTGATGATCCCTTCCAGAAAAGCTTGCCTTTTT11.6129032258064515No Hit
GAGATATATAGCAAAGAGATACTTTTGAGCAATGTTTGTGGAAGCGGTAT11.6129032258064515No Hit
GGTTACGAGGCAATCAGCAGTCGGATATCGTTAAGAATGCAGCAAAAAAA11.6129032258064515No Hit
GTATCATAGGATGCATCTGATAAAATAGGTGAAAAAGCGCTCCTAGAGGG11.6129032258064515No Hit
CATATCACCTGCGTTTCCCTTAAACTATCGGTTGCGGCCATATCTACCAG11.6129032258064515No Hit
AACATATTTTGTGTTTTCCAATGAATTCATTATTACCCAACTATCTTCAA11.6129032258064515No Hit
ATTATGTATAATGGGTTGAAGATATAGTCCAAATATAATTGAAAGATTAT11.6129032258064515No Hit
GGTTGGGCCGACGCGGTTGCTTGAATGTGGCCCCTGTAAGAAACTTTATT11.6129032258064515No Hit
CCTTGATATTGTGTGCCTCATCCAGAATAACTCTATAAAAGTCAATATTA11.6129032258064515No Hit
TATATAAATTACATAGCTGCAAGTCATGAGGAACAGAATAACCCCCAAAG11.6129032258064515No Hit
CTGCCAAATGATACCTAGAATTTGTGGTGGTATCCCATTGGTAACATGGG11.6129032258064515No Hit
GATTTATACAATGAAAGAGCCCAAAGCTCCTGATAACGATGAAGCCGGAA11.6129032258064515No Hit
TGTCTGGACCTGGTGAGTTTCCCCGTGTTGAGTCAAATTAAGCCTCAGGC11.6129032258064515No Hit
ATATACGATGCCACCCATTGCGATGAGGTCGTTGTTTGTATTGCTTGCCT11.6129032258064515No Hit
GCTGTAAAGTCTCTACAAATTGAGATTCAATTCTTCGCAGCTTTAGAGAC11.6129032258064515No Hit
TGTTAAAAGGGGTTGGCTTGTGGCTGAACCGTCTGATAGATTTAATGTTG11.6129032258064515No Hit
TTCTTCAGGTGTCATCCCAGCGAGAAGCCTATTATTTATCATTATTCTAT11.6129032258064515No Hit
AATTTATATTATCCAATAATCAAAAAACCAAACAATTAATTTTCAAAGAA11.6129032258064515No Hit
TAATTGTTTAATGTAAATAATCATGAAATAGAATGAAATTTTGAGCAGCA11.6129032258064515No Hit
CAATAAATTAATACTGGATTCAGACTCTTCCGATGATGAGATATTACCTC11.6129032258064515No Hit
CATCAGATTATATCGCGTTGACTTTAGTCAGGTTGAGAATGTCTTGCAAG11.6129032258064515No Hit
ATTCTAACAGGGTTCAAACCAGCAATTCTAGCAGCATCAGCAATGTTGTA11.6129032258064515No Hit
GTTTAAGTACATGTATTAGGCTTGACTTTCTTCACCCTCCACTTTTATTG11.6129032258064515No Hit
GTAAAAGAACCATTGATTTTGATTTTCAGTGTGAATACAAACAATCAAAT11.6129032258064515No Hit
GCCAAAAACATGCAAAAAAAGCAAACTAACAACCCAATGCCGACAATACC11.6129032258064515No Hit
CTTGGTGATACGGACTAACAGGTATTCATATGGGAAGATATGAAAATTGT11.6129032258064515No Hit
GATTTATAGATTTCGTCATAATTCTTGGGAATGTCAGAAAGCCTTTCACG11.6129032258064515No Hit
GGATGGAAGAAAGTCACAAAAAGGAGTTTCCCTCTTATATCTTACTAATA11.6129032258064515No Hit
GGCATAATGGTTATATGCCGCCCGTCTTGAAACACGGACCAAGGAGTCTA11.6129032258064515No Hit
GTTTGCGGCTGGCAGGCGGTTGGGGTGAAACGTCAGGCGGTGGTGGTGAC11.6129032258064515No Hit
GATCACTACAATGCCGTTGCTGCTTATTGCAGCGTAAGTGAGCTTCATTC11.6129032258064515No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATC50.070.032
TGATGAT50.070.020
CGTCAAA50.070.044
TTGCAAA50.070.054
ACGCTCA50.070.09
GTCATCT50.070.033
ATCTTGA50.070.036
GCCCAAG50.070.063
GATACTT50.070.024
AACGATT50.070.049
AAATCGC50.070.058
TGCGTCA50.070.030
CCAGTGA50.070.016
ACTTGCG50.070.027
CACGCTC50.070.08
CGCCCAA50.070.062
TGCAAAT50.070.055
ATGATAC50.070.022
CATCTTG50.070.035
CCACGCT50.070.07
Produced by FastQC (version 0.11.5)