FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-G07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-G07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817902
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG11800.016.3183211
TATACTG17050.014.9846585
CTAATAC11950.014.3508133
CCGCTCG2454.268426E-614.2878569
GTCCTAA6700.014.1085951
TAGACCG6400.013.6712545
GTATATG15600.013.4654671
CCTAGCG4002.2646418E-913.12440530
TAGGTAG6450.013.0226655
TAGTCAG5650.013.0107989
GTATATA29050.012.8953291
GGTAGTC7100.012.81631857
GTATAGG8850.012.65906051
TCTATAC12900.012.4800543
TACACAG8550.012.2801455
ACCGTGT3751.8191349E-712.1327838
ACGGTGT7800.012.1148348
GTATAGA13400.012.0184331
GTCCTAG3852.5494955E-711.8216311
GTAGCAC7450.011.7444333
Produced by FastQC (version 0.11.5)