FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-F11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-F11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4305204
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC7400.017.97326364
CCGCACC7700.016.81845365
CGCACCT7450.016.44340966
TATACGG14200.016.2667355
CTAATAC20900.016.2433383
GTATTAG18150.016.2004891
TAGGTAG10800.013.6103825
TACGGTG14400.013.6103817
ACGGTGT12900.013.56516558
GTATAGA20600.013.4241061
TAATACT32400.012.9622684
GTATATA45550.012.833751
ATACGGT15900.012.7666116
TCTATAC19250.012.7267393
ACTATGC18200.012.499338
GTATAGG16000.012.4703991
CATATAC26650.012.0820453
TATACAG27400.012.0066555
ACTGTGC9950.011.9591588
GTATAGC12900.011.9395641
Produced by FastQC (version 0.11.5)