FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-E11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-E11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903061
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG12750.018.1219961
TAGGTAG5850.017.3501075
GCGCGGC2352.772107E-614.89488112
CGCGGCC1901.13403905E-414.73809413
GTCCTAA6950.014.60782051
CGTCCCG1450.00473954114.4839889
TACACCG5200.014.1343565
TATTAGG16500.014.0002232
GTATATA34050.013.5715381
TCTATAC15250.013.5412023
CGGTCGG2607.905683E-613.46129169
TATACTG18600.013.3599685
CTTACAC6950.013.0937343
CCGTCCA4052.7212081E-912.9640649
CTGTGCG3254.2381544E-712.9241739
GTATAAC13550.012.9182281
TATACGG10600.012.8771225
CTGCGCG1950.002300268312.565179
ACCGTCC5357.2759576E-1212.4296778
TAGACAG9750.012.2049045
Produced by FastQC (version 0.11.5)