FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-E10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-E10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3669781
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG16600.017.5041641
CTAATAC15650.016.5489483
TCCGCAC7100.015.28199464
CCGTGCG2101.5817051E-515.0017289
GTATAGA17000.014.4151941
CCGCACC8050.014.3481165
CTAGGAC5450.014.1279523
CGCACCT7800.013.91072366
ACCGTGC3555.997208E-913.8021518
GTATATA34300.013.3705261
TATACGG12350.013.3191965
ACTATGC16850.013.2931278
TAGGTAG8800.013.1243665
ACCGCGC3002.4425226E-612.8327148
CATATAC22900.012.5323173
ACGGTGT10750.012.3714968
TATACAG21850.012.3335015
TACACCG7100.012.3233495
TAATACT27850.012.3153854
GTATAGG13100.012.2931
Produced by FastQC (version 0.11.5)