FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-E07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-E07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080329
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG8150.015.8885025
GTATTAG14050.015.6972271
GTATAGA15500.014.9063371
TCCGCAC5200.014.80765964
CTAGGAC4351.8189894E-1214.4820183
GTCTAGG6100.014.3472571
CTAATAC15450.014.044593
ACCGCGC2801.124874E-613.7490768
CCTAATA15300.013.7250132
CCGTGCG1550.00737110313.5503359
ACTATGC12750.012.90109358
TATTAGG14200.012.8164562
GTATATA33900.012.7017661
CCGCTCG1950.002299306712.5659099
CGCACCT6150.012.52070366
GGTAGTC7050.012.4105147
TATACTG17800.012.3868075
CATATAC20950.012.1950823
CCGCACC6950.012.08649965
GTCTATA8700.012.0714851
Produced by FastQC (version 0.11.5)