FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-E03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-E03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2537919
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT4400.016.70489966
CCACGCG1554.0544718E-415.808039
TCCGCAC4500.015.55588464
CCGCACC4500.015.55588465
GTATTAG10400.015.1481321
CAGTCCG1400.003753091415.0014989
TAGGACT4450.014.9429594
CCTACAC4351.8189894E-1214.4822093
TAGACCG6350.014.3301645
CTAATAC12800.014.218213
TCCTAAG6950.014.10073952
CTAGGAC4505.456968E-1213.9994683
ACTCCGC4555.456968E-1213.8456288
ACGTCCC3051.9593062E-713.7699695
GTATAGA10800.013.6146171
TACACTG8550.013.0989175
TAGGTAG5103.6379788E-1213.038725
CTACCCG2254.7538584E-412.4439725
TATTAGG12450.012.3694952
GTCTTAC7100.012.3271181
Produced by FastQC (version 0.11.5)