FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-E02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-E02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938665
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG8450.018.6474021
TAGGTAG4350.016.8958365
CGCACCT3301.2551027E-1015.90923766
TATACTG13350.015.4675095
TAGGACC4350.015.2867094
CCGCACC3704.3655746E-1115.13527565
CTAGGAC3051.213084E-814.9177863
CTAATAC8700.014.8848113
ACTATGC7450.014.5631418
TATACGG6500.014.5378465
CCGGCCG1450.00473792314.48438614
CGGGAGT2001.75534E-413.99940731
ACGCCCG2052.1614945E-413.66007111
GCTCGGT1800.001258749313.6126411
ACCGCGG1800.001260220213.61053518
CAGCGTG3951.869921E-913.2926329
TCCGCAC3701.0697477E-813.24336564
CTATACT7400.013.24268154
GTATAGG6350.013.2342711
CTCCTAG2405.5439927E-513.1308781
Produced by FastQC (version 0.11.5)