FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-D07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-D07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3356851
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG8300.016.8671725
TATACGG10700.013.7380755
GTATTAG13250.013.4748731
GTATAGA14700.013.0983231
CTAATAC14200.013.063153
GTATAGG12650.013.0070161
ACCCGCG3254.2441025E-712.92284933
GTATATA31650.012.7202151
CTTACAC7850.012.4838563
TATTAGG16000.012.4690882
TATACAG17800.012.3874215
TACACTG10650.012.1594095
TAGGTAG7600.011.9734745
TAGACAG10550.011.9429175
GTGTATA12700.011.8531871
TCGTGCC4501.3029421E-811.6669839
CCGCACC6351.8189894E-1211.57459965
TACACCG6055.456968E-1211.5700445
GTACTAG9450.011.4841861
CGCACCT6800.011.32349866
Produced by FastQC (version 0.11.5)