FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences804066
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG450.004304722431.1155051
GAGCGCG750.001831048323.336639
AGGTGTA5850.019.74269570
TCCCTCG900.005276895619.4435627
CGTTCGG1107.467348E-419.09360712
GGCCCAC1501.4961439E-518.66582154
GGAGCGA950.007202892618.42365510
GCCGACC950.0072106518.42021870
GCTGCCC1551.9841704E-518.06369870
GCCGGAC1000.00968003817.50247411
CCCTCGC1000.00969044117.4992078
GTCTAGT2108.561856E-716.6690221
ACTCCGC2351.5419027E-716.3822368
TGGACCG1300.002290686616.1531145
ACCGCGT2201.394219E-615.90837117
CTAGACC2009.960388E-615.7492874
CCCTGCC1350.002942802615.5577539
CCTGGAC1350.002945243615.5558193
TAGTATA2704.3939508E-815.5548514
TCTAGTA2051.2579047E-515.3661122
Produced by FastQC (version 0.11.5)