FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-D02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-D02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061268
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTACAACTCCGCATACCGCGTCGCCGCGTCGCCGCGTCGCCAAAAATT23950.11619061664955746No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT5200.026.9289471
CGCACCG6200.025.96836336
TCACGGG6100.025.81903619
GCGCACC6250.025.75936535
CGGGCCG6500.024.76862122
ACGGGCC6450.024.41800521
CACGGGC6750.024.36977220
CCGGAGC6900.023.8411540
CGCGGGA2650.023.77299956
TCCTTGC7250.023.1718438
GGGCCGT6950.022.66131223
GGCCGTC7300.022.53369124
GCCGTCA7550.022.25110625
AGCGCAC7650.021.96024534
GTCCTAA3400.021.622361
ACCGGAG8050.021.3048639
CTCCTTA8950.020.3396281
CCGTCAC9150.019.89022326
TCCTTAG8650.019.8265842
GTCCTAG2504.1836756E-1119.6042751
Produced by FastQC (version 0.11.5)