FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-C11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-C11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302990
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAA5050.015.9442591
GTATTAG8800.015.514931
TATACCG5650.015.486255
CCTATAC6050.014.4623663
TATACGG7800.014.3585345
TAGGTAG6150.014.2272055
CTATACT9450.014.0736424
GCCGAGC1750.001018727313.999578
TCGGGGG2303.7336787E-513.69552916
CGGTCGG2102.652495E-413.33408213
GTCGGGG2153.2359138E-413.02426915
GTATATA21050.012.9721021
TATACTG14150.012.8617965
TCTATAC9850.012.7914863
CATATAC13450.012.7505383
CTAGGAC3356.127575E-712.5369293
AGGACAG6150.012.5199415
GTATAGA9750.012.2079341
GTCTAGA5501.2732926E-1112.0936891
GTCTATA7850.012.0409831
Produced by FastQC (version 0.11.5)