FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-B11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-B11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995631
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG2751.6007107E-1017.8221241
GTGTAAG5800.015.6931281
GTATATA24800.015.3864681
TAGGTAG4600.015.2169455
GTATTAG8300.014.7623011
GTCTAGA5750.014.0030981
ACCTAGG2253.0289704E-514.0030971
GTAGGAC3001.6002195E-713.9999413
TATTAGG9300.013.9250192
GGCCGGG2052.1627637E-413.659162537
TATACAG14200.013.555945
TTAGGTA7600.013.3548714
TAGGACA5600.013.1246164
TATACGG8000.013.1246155
TCCGCAC3558.8959496E-812.81684664
TATACTG12350.012.7526625
GTATAGG6350.012.679971
GTATATG13750.012.4754871
CCGCACC3651.2781311E-712.465765
ACCATGC6500.012.3842528
Produced by FastQC (version 0.11.5)