FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2781127
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG11050.015.525141
CTAATAC11550.015.15154653
TATACGG8250.014.4237545
CGCACCT5150.014.27187466
GTATAGA12450.014.0605511
GTATAGG9050.013.9269371
TATTAGG12000.013.4174182
TACACAG8800.013.1245565
GGCGTCG4103.272362E-912.80444432
GTATATA24650.012.7828371
TATACTG15900.012.7668635
TCCGCAC5601.8189894E-1212.50002664
CATATAC15650.012.3003443
TATACCG8300.012.22855
GACCGCG2305.720234E-412.1735017
GTGGCGT5501.2732926E-1112.09049930
GTGTAAG7250.012.07267951
CTTAGGG3802.1580308E-711.9743542
ATACGGT9700.011.9068136
CTAGGCA5308.54925E-1111.886394
Produced by FastQC (version 0.11.5)