FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-A04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-A04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2306228
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG10750.017.2618121
TAGACCG5800.014.4818265
TTAGGAC5200.014.1343183
TAGGTAG5300.013.8670325
ACCGCGC2052.165016E-413.6576578
TATACGG6750.012.9621275
GTATATA20900.012.8992191
TAGACCC3801.5561454E-812.8939074
TCCGCAC4406.87578E-1012.72755764
GACAGTG6950.012.5891177
CGAATAG12850.012.52837670
TATACTG15100.012.515755
TAATACT18500.012.48568254
CTAATAC11500.012.4779983
GTCCTAG3103.5091944E-612.4236721
CCGCACC4551.1659722E-912.30796765
ACTATGC10250.012.2918918
TTAGACC6600.012.1961844
CCTTAGG5206.002665E-1112.1195961
TAGAGCA7800.012.1146044
Produced by FastQC (version 0.11.5)