FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-A03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-A03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558105
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC4650.018.0642364
TAGGTAG5400.017.4997225
CCGCACC4800.017.49972265
CGACGTG4300.017.0927532
CGCACCT5150.016.9900266
CACGCAG5000.016.79973416
TGCGACG5100.016.47032530
GGTGCGA4750.016.21026828
GCGACGT5400.016.20344531
GTATTAG12100.016.2031571
GTGCGAC4750.015.47343729
GTATAGA11850.015.363211
TATACAG17800.015.3368345
GACGTGA5050.015.24728333
CTAATAC11900.015.0003473
ACCCGCG2352.7746992E-614.89337933
CATATAC17500.014.6003383
CCATGCG5350.014.3937019
ACTATGC10750.014.3253548
TCGGGGG2202.456811E-514.31795534
Produced by FastQC (version 0.11.5)