FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n02_AP1-A02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n02_AP1-A02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4663851
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG7050.016.87884170
CTAATAC19700.015.989633
CGCGTGT6400.013.67139470
GTATTAG17050.013.1401861
TCCGCAC8350.012.99397564
TAGACAG15550.012.6039255
CCTAATA21950.012.5971782
TATACTG27050.012.4208735
TCTATAC20400.012.1812073
ACTATGC18150.012.1482048
TAGACCG9850.012.0806655
CTATGCA15950.012.0710019
ACAGCGC7700.011.8176398
CTTACGC18200.011.53805670
ACAGTCC8500.011.5288848
CTAGACA14600.011.5063234
CCTATAC13700.011.4961943
ACAGTGC11300.011.4596528
TATACCG12550.011.433745
GTATATA36200.011.4108861
Produced by FastQC (version 0.11.5)