FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12023138
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG382240.31792032995046715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG25400.014.882741
CGCACCT14100.014.20321966
TATACGG20450.013.9844265
TCCGCAC14750.013.55425764
CGCGGGA4709.094947E-1213.43670956
TATACAG38950.013.2519055
TAGGTAG14900.013.1076795
CCGCACC15450.012.93906765
TCTATAC27050.012.8931373
GTATAGA27850.012.6937241
CTATGCA25300.012.6819489
ACTATGC29100.012.5838918
GTATATA64550.012.3089911
CTAATAC35650.012.0329323
CTATACT28550.011.97137454
TATACTG37900.011.6866115
GTATAGG20700.011.4982421
CTAGGAC9850.011.3302543
ATACAGG24850.011.0872756
ATACAGT35650.010.9567716
Produced by FastQC (version 0.11.5)