FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_Gbc_c83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_Gbc_c83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2789277
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC5750.016.43455964
CCGCACC6400.016.40602765
CTAATAC13650.016.1539153
TCTATAC13800.015.9783313
GTATTAG12100.015.6204621
CATATAC23500.014.5958083
TATACGG12400.014.3952245
CGGCCCG3655.7480065E-1014.38336814
CTATACT15750.014.2222844
TACACCG7150.014.1958665
TACAGCG5300.013.8677367
GCGCTCG2303.734394E-513.69546733
GCGGGGC2659.619733E-613.20760426
TACGGTG10600.013.2073687
CGACGGG3203.5138146E-713.12505749
CGGGGCG3203.5138146E-713.12505727
CGCACCT6950.013.09452166
TAGGTAG7000.013.0000565
CTCTATA14400.012.8820012
ATACGGT12500.012.8800566