FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_Gbc_c77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_Gbc_c77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3846070
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC4750.015.4732938
ACGGTGT11500.014.6083268
TACACAG11400.014.1231845
CTAATAC18450.014.0383163
TCCGCAC6800.013.89670864
TATACAG25050.013.27380855
TAATACT32450.013.2669194
CATATAC25900.012.9733213
ACCGCGC2701.1669084E-512.9626358
TATACGG13750.012.7276135
CCGTGCG2254.754337E-412.444139
GTATAGG12700.012.4020671
CCTAGGA5104.3655746E-1112.3534332
CGCTCGA3407.335402E-712.35262910
CCTATAC12850.012.2571373
TACGGTG12900.012.20931157
TAGGACC6600.012.1972964
TCTATAC16800.012.0836553
CGTAGAG16000.012.03172870
GTATATA41450.011.9908271
Produced by FastQC (version 0.11.5)