FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_Gbc_c74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_Gbc_c74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4923650
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19950.016.492911
TATACCG13400.014.1056315
GTATAGA21250.013.507261
TATACAG23650.013.1723225
CTATACT20500.012.8059234
TATATAC43400.012.6624533
TAATACT36250.012.2630714
GAGCCCC6000.012.2501370
CTAATAC20300.012.2425033
GTATATA38900.012.2377521
TATACTG25050.012.1566825
CCGCGCG3802.1614687E-711.9734469
TATTAGG19900.011.9608972
TCTATAC18200.011.5395213
CCTAATA20650.011.5264822
TCTAGAC12300.011.383163
ACTCCGC10200.011.3233048
TATTATA49250.011.2294582
GTCTATA15350.011.1737781
TCCTAAG12250.011.1438812