FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_Gbc_c71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_Gbc_c71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1688381
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC3950.017.72102565
TCCGCAC4050.017.28346864
TATACTG12150.016.9954095
CGCACCT4000.015.75095866
GTATTAG7850.015.6055841
GCGACTC1350.00294733815.55558234
ACTCCCG1807.1557304E-515.55512220
ACGGTGT6100.015.491378
CTAAGCC3452.4556357E-1015.2169673
CCCCGCT2151.9770483E-514.65075465
GTCCGGC2403.4512395E-614.58292611
CTAATAC10000.014.3495993
CTCCCGC2001.7544726E-414.00002321
GGCGCCG1500.005936690614.00002325
ACCCGCG1500.005936690614.00002333
GCGCACT2001.7549138E-413.99960811
GTATAGA9050.013.9230881
ACCGAGG7100.013.80365870
GTATATA22900.013.7558891
CCTATAC6400.013.6714943
Produced by FastQC (version 0.11.5)