FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_Gbc_c69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_Gbc_c69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1200212
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC901.9288015E-423.33358438
ACGCGCA1451.1171336E-519.3105539
TGACGCG1351.4123916E-418.14834237
GCGACTC1602.6073289E-517.50018934
AGTCCGA1200.001341393617.49945810
TATACGG5300.017.1699945
TCCGCAC2108.585939E-716.66615164
CCGCACC2151.0978347E-616.27856665
CTTAAGG2401.9603067E-716.0418383
TAATACT10800.015.2316454
ACCGTGC1400.003754824214.9995358
TAGGTAG3051.21108314E-814.9181925
GTATAGA6900.014.7109161
CGAGTGT1450.004739728314.4829146
CCTTGCG2901.05656E-714.482319
TATACTG9200.014.0762395
CCGTGCC1500.005936654313.9995679
CGCACCT2801.121718E-613.7507266
ATACGGT5100.013.7256376
CTATACT6400.013.6720224