FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_Ap3-H12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_Ap3-H12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433772
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC2600.021.53773165
TCCGCAC2603.6379788E-1220.19162464
TAGGCGC900.005277459519.44446421
TAGGTAG3550.018.7343735
CCCTAGG1000.0096836717.5024591
CGGTCCG1000.00969145117.50001756
TATACAG13450.016.6560345
TAGATAG5600.016.2517175
CGCATGG4400.015.90910547
CTAGGAC2653.491914E-815.850733
GTATTAG7750.015.8086741
CCGCATG4650.015.80646946
ACGGTGT5350.015.7004028
GAGCGCA1350.00294774615.5550299
AGGGTAG5200.015.48024516
CTAGGGT2051.2582159E-515.3674774
ACCCCGC4850.015.154654543
GGCGTCG1859.036745E-515.13515132
GTGCGAC4400.015.11312429
ACAGCTA7650.015.09752758
Produced by FastQC (version 0.11.5)