FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_Ap1-E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_Ap1-E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563786
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGC1502.4805558E-823.3328238
TAGGTAG3900.021.5400545
ACATAGG3900.020.64057431
TATACGG6400.018.5951255
GTATTAG8300.016.8697951
CCGCACC2702.415618E-916.85148265
CGTAGAC1957.8337325E-616.155043
TATACAG11350.016.0364285
GCGCTCG1554.0569901E-415.80610533
TACGGTG5450.015.4129987
TCCGCAC2755.5142664E-815.27239164
TATTAGG9200.015.2190032
GTAGGAG4600.015.21754470
GGTAGTC5300.015.1888327
ACGGTGT5300.015.1883478
ACAGCGC2553.904588E-715.0977098
TCCTAGG2101.5806898E-515.0015912
AGGCCCG1400.00375527514.99967110
CTAATAC7550.014.8355343
GTGTAAG5000.014.7020271
Produced by FastQC (version 0.11.5)