FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3.F07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3.F07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences339989
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG1900.036.84210670
TACGTAC400.00241704535.00000412
TCGGGGG601.21403245E-535.070
GAGAGGC3100.031.61290470
GGGTAGG450.004304236731.1111091
GGCTCGG450.004304236731.11110970
CCAGAGC1251.05501385E-1030.870
GATCGGG3700.030.27027170
AGCGGGG853.94822E-628.8235370
CGACGGG500.007203341527.9999988
TTACGAC500.007203341527.9999983
CTCCGAC657.9477485E-426.92307770
TAGACTT657.9477485E-426.9230775
GAGCGGG1056.753089E-726.66666869
CACGAGC2100.025.00000270
GTACCCA851.3063723E-424.7058836
ACGTGCA750.00183038923.3333347
CCGCGCG750.00183038923.3333349
GGATCGG5150.022.42718369
ATCTAGC1102.9156507E-522.2727261
Produced by FastQC (version 0.11.5)