FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-H01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-H01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2712502
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG2100.026.66686454
CGCGGGA2555.4569682E-1119.21582856
AACGCGC2952.3646862E-1117.7967452
GCACGCG2401.0317308E-817.49948319
GCGCGGG3156.184564E-1116.6667955
CCGTCCG2351.5449586E-716.383131
GTATCCT11300.015.7977411
TCCTTGC17300.015.7798828
CCCTGCC6800.015.44072159
CCTTGCG8950.015.2509489
CCCTTGC8750.015.1995538
TAGGTAG6000.015.1676175
CGTCCGG2553.9063707E-715.0981532
CCCTTAG7900.014.1783661
TATACTG16300.014.1726675
GTATATG19250.014.0011361
ACGCGCG3504.9058144E-914.00010353
CACGCGC3051.9592699E-713.77008520
GACGCGC2801.1237862E-613.75010138
CCTCCTA5350.013.7394321
Produced by FastQC (version 0.11.5)