FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-G12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-G12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1829366
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG4750.017.6847765
GACGTAG7900.015.94987870
ACGGTGT5800.015.68938
CCTAGAC3653.45608E-1115.3429573
GTATTAG8800.015.1145341
CCTATAC7250.014.9659973
GTCCTAT4251.8189894E-1214.8244091
GGTAGTC4750.014.7365087
CATATAC14700.014.5242753
CCTAGGG1450.004740316414.4832223
TCCGCAC3151.8555511E-814.44411864
ATACGGT8200.014.0858176
ACATAGG4252.7284841E-1114.00006631
CACCGCG1500.00593682414.00006523
TACGGTG6500.013.9996837
CCTTGCG4252.7284841E-1113.9996829
GTATAGA10550.013.9344771
TAGGGCG1550.007375047513.5488215
TCCTATA9650.013.4201192
CCGCACC3405.0584276E-813.3820565
Produced by FastQC (version 0.11.5)