FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-G11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-G11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1887926
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACAC4150.016.8696273
TACACAG6850.016.3524555
GTATTAG9450.016.298381
GTATATA25050.015.2314891
TCCGCAC3503.0559022E-1014.99953664
TAGACTG4750.014.7387275
TAGGTAG3604.656613E-1014.5851985
CTAATAC8850.014.239113
TCGGGGG1750.001018406213.99993734
CGCACCC1500.00593832113.99956663
CGCACCT4051.8371793E-1013.82746466
CCTAGCC1800.001258580613.6128523
CTACACA5700.013.51054
TATACTG12700.013.5056645
AGTCCGG2607.903209E-613.46112210
TCCGGCC2354.5702123E-513.4038412
CCGCGCA1850.001549226813.2428329
GTCCGGC2405.568802E-513.12459311
CCGCACC4002.2610038E-913.12459365
CTAGGAC3254.2332977E-712.924733
Produced by FastQC (version 0.11.5)