FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-G10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-G10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1292276
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5650.023.5400711
TAGGTAG3650.022.0541745
GTCCTAG1558.750085E-720.3227941
TAGGACT2601.4624675E-917.4995064
TAGTCAG3700.017.0265469
ACCGTCG2954.3655746E-1016.6097018
CTAATAC6150.015.9345093
TTAGGTA5600.015.6245594
CCGTCGC2503.118421E-715.3995679
TATACAG10250.015.365425
ACATAGG4151.8189894E-1215.18088231
CCTAGCG1901.1339846E-414.73699730
TAGACCG4101.4551915E-1114.5117865
TACGGTG4101.4551915E-1114.5117867
TATTAGG7300.014.3831572
ATTAGGT7400.014.1887893
CATAGGA4453.6379788E-1214.15745232
GACCGTC3252.7965143E-813.9996067
TATAGTG6750.013.9996055
CTAGGAC2253.0344647E-513.9996053
Produced by FastQC (version 0.11.5)