FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576138
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC1356.0261973E-620.74105838
GTATTAG6900.016.2326491
TCCGCAC2957.8071025E-915.42347764
CGCGCAT2051.2594497E-515.36609240
TACACAG5700.015.3506275
CCGCACC3201.3842509E-915.3122565
ACGGTGT6100.014.9177898
ACGCGCA1901.1341882E-414.73706939
ACTATGC6950.014.6040788
TATACAG9000.014.3886555
GTATAGA7400.014.1898581
CCGACCC1500.00593591914.00021644
CCGGTTA2253.0340518E-514.00021668
CCGACCG1500.00593716713.99977218
CCTTGCG4750.013.9997729
TGACGCG2052.1628679E-413.65874837
TAGGTAG3851.2769306E-913.6361425
GTAGCCC1550.007376811513.5481663
CTACACA5250.013.3331174
CTAGGAG1850.001548857813.2430273
Produced by FastQC (version 0.11.5)