FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706587
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC5850.016.153843
GTGTAAG5300.015.84905051
TATACCG5450.015.4128375
TACACAG5900.014.83050255
GATCGCG1500.00593680813.9999947
CCCTACA2801.1231841E-613.7499932
CTGATCG2303.7332964E-513.6952449
GTATTAG6950.013.5971161
TAGACTG3801.5537807E-812.89473155
ACCGTGT2456.755371E-512.8567618
TCTATAC8350.012.5748453
CAGGCCC2801.6949083E-512.4996289
TCCTATA6500.012.384612
GTTCCCC2559.818778E-512.3529371
AGCGTCC2000.002782984212.2496378
TATACTG12600.012.2222185
TTATACT13500.012.185184
GTATATA22200.012.1396351
CTATACT9350.011.9786054
CTATACA8800.011.9318134
Produced by FastQC (version 0.11.5)