FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-F10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-F10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705673
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG8400.017.501571
CGCACCT2753.079549E-916.54596766
AGGCGCG1300.002292279916.15292718
TCCTATA8050.015.2187572
TAGGTAG4009.094947E-1214.8763325
CCTTGCG5000.013.9992029
TCCTTGC11500.013.3905428
AGCGCAC2659.620591E-613.20679510
GAGTCCG2952.0211555E-613.0501049
TCTAGAC4601.0004442E-1012.9359433
TCCGCAC3601.0681288E-712.63816964
TATACCG5551.8189894E-1212.6137445
ACAGTGC3952.6702764E-812.4043568
TACACAG4802.055458E-1012.3969445
ATGCTAG6900.012.1750041
TATACGG8100.012.099855
GACGCGC2356.84759E-411.91526338
GCTATAC5004.0563464E-1011.9010673
TAGGGTG2952.8903916E-511.865475
CTTCCTA6200.011.8559021
Produced by FastQC (version 0.11.5)