FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-F06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-F06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1940320
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG8400.015.8340451
TCCGCAC4100.015.36535664
CCGCACC4100.015.36535665
CGCACCT4009.094947E-1214.87528566
GGTAGTC4403.6379788E-1214.3177177
GTCCTAT5450.013.4868451
CCCTATA5750.013.3912152
TAGGTAG4751.2732926E-1113.263075
GTATATA27650.013.1651481
GCGCGGG1950.002300929312.56434355
ACTATGC9300.012.4189538
CATAGGA6050.012.14899332
ACAGCGC2601.1775304E-412.1149918
CTAATAC10450.012.0573373
GTATATG14600.011.7471031
TAATACT18550.011.6980354
ACATAGG4802.7066562E-911.66688931
CCTAATA12050.011.618182
TTATACT15800.011.5189114
CTTAGAC6700.011.4924623
Produced by FastQC (version 0.11.5)