FastQCFastQC Report
Tue 18 Dec 2018
H7HVFAFXY_n01_AP3-F04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7HVFAFXY_n01_AP3-F04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1606943
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCG1551.9858202E-518.06407523
GCTCGGG1000.0096916317.50011643
CCGTGCC1200.001341602917.4995739
TCCGCAC2056.676164E-717.07275264
GACTGGG2151.0983276E-616.2786717
CCGCACC2051.2599565E-515.36547765
ACAGTCG1450.004741762814.48240413
GTATTAG6750.014.0000931
GTATAGA8250.014.0000931
TAGGTAG3801.0495569E-913.8158825
TATACGG6900.013.6957445
CGCACTC2052.1636413E-413.65820212
GGCGTCG2607.90093E-613.46120832
CGACGGC1600.0090958613.12549645
ACGACCG1600.00909772913.12508839
CCGTTCG1600.00909959813.1246799
CCTACAC2701.1655211E-512.963053
AGTGTGC3254.2398096E-712.9227618
CGCACCT2456.7486544E-512.85802866
CATATAC12050.012.7801693
Produced by FastQC (version 0.11.5)